80 (Genbank accession numbers #PP584053-PP584054). All but one individual shared Haplotype CcaNC01, and the secondary haplotype CcaNC02 found in that single individual sampled in the RP area differs from the main haplotype by one bp. In consequence, haplotype and nucleotide diversity were extremely low overall (h = 0.031; sd = 0.030 and π = 0.00004; sd = 0.0001, respectively) and no genetic difference was detected between the two areas at the mtDNA level (FST = -0.00551; P = 0.99). From the original 38 individuals sampled in each nesting area, 35 and 30 samples were successfully genotyped for a minimum of seven microsatellite loci and thus retained for the RP and the GLS areas respectively (Tableau 6). The mean number of alleles per locus was 7.8 ± 1.9, n = 9 and 8.1 ± 2.7, n = 9 for RP and GLS samples, ranging from 4 (Cm72) to 12 alleles (Cc5F01). Mean expected heterozygosity across loci was similar for both RP and GLS samples (0.794 ± 0.040, n = 9 and 0.783 ± 0.061, n = 9, respectively). Pairwise comparison based on microsatellite diversity showed no evidence of genetic differentiation between the RP and the GLS (FST = -0.00195; P = 0.61). Tableau 6. Genetic diversity of loggerhead turtles from the RP and the GLS areas genotyped at nine loci. n = number of genotyped individuals per locus; Na = number of alleles; HWE = Hardy-Weinberg Equilibrium; P = pvalue. Locus n Na Observed Heterozygosity Expected Heterozygosity HWE (P) RP GLS RP GLS RP GLS RP GLS RP GLS Cc7 35 30 8 9 0.914 0.833 0.850 0.831 0.457 0.246 Cc1F01 35 28 9 9 0.857 0.857 0.777 0.808 0.342 0.398 Cc7B07 33 29 10 11 0.758 0.828 0.834 0.854 0.283 0.410 Cm72 31 23 4 4 0.548 0.609 0.726 0.658 0.315 0.099 Cc117 35 30 8 6 0.914 0.833 0.812 0.771 0.514 0.965 Cc5F01 35 29 10 12 0.771 0.828 0.817 0.812 0.645 0.370 Cm84 35 28 7 6 0.685 0.786 0.778 0.731 0.209 0.088 Cc141 35 30 7 6 0.771 0.767 0.752 0.756 0.146 0.998 Cc2G10 35 28 7 10 0.914 0.750 0.798 0.828 0.582 0.006 mean ± sd (n = 9) 34.3 ± 1.4 28.3 ± 2.2 7.8 ± 1.9 8.1 ± 2.7 0.793 ± 0.123 0.788 ± 0.076 0.794 ± 0.040 0.783 ± 0.061
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